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Enzymatic reactions were performed in 96-well plate format (Greiner Bio-One, 655101) with 190 l of reaction mix and 10 l of enzymes diluted in 10 mM sodium phosphate buffer pH 7.0 at the following concentrations: 1.36 g ml 1 (LacAnc100) and 0.81 g ml 1 (LaccID) for ABTS, 1.7 g ml 1 (LacAnc100) and 0.81 g ml 1 (LaccID) for DMP and guaiacol and 6.8 g ml 1 (LacAnc100) and 8.1 g ml 1 (LaccID) for sinapic acid
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Boiling and prolonged cooking may decrease glutathione levels, while steaming or lightly cooking vegetables may help preserve more of their beneficial nutrients
10.1385/1-59259-419-0:133 24 JoshiS.ErginA.WangM.ReifR.ZhangJ.BruceJ
